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Southern Blotting |
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| Electrophoresis of DNA is carried out in a neutral agarose gel system. Prepare a 0.8-1% agarose gel containing 1x TAE buffer. Ethidium bromide can be added to ... |
/ United States
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Conjugation of monoclonal antibodies |
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| In this series of web pages, protocols, notes, and various illustrations are given to aid in the conjugation of proteins--principally monoclonal antibodies--to... |
/ United States
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Inverse PCR for PAC-end sequencing |
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| Goal is to generate PCR fragments that contain the ends of PAC inserts that can be sequenced. For inverse PCR we cut the PAC once in the vector (near outward ... |
/ United States
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Cell Culture |
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| Remove ligament from animal using as sterile technique as possible. A one inch
segment from an older 270 day old fetus will be more than sufficient. |
/ United States
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Immuno Techniques |
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| In mildly acidic conditions, the addition of short-chain fatty acids such as caprylic
acid to serum will precipitate most serum proteins with the exception of... |
/ United States
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